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Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. It is a metachromatic dye suitable for a wide variety of histological staining procedures.
Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in DNA and RNA. It has found wide applications both as vital staining in living tissues and as a special stain owing to its metachromatic property.
- Gokul Sridharan, Akhil A Shankar
- 10.4103/0973-029X.99081
- 2012
- May-Aug 2012
El azul de toluidina o cloruro de tolonio (según el INN), a veces abreviado como TBO) es un colorante catiónico (básico) metacromático de color azul, utilizado en histología y algunas veces en el ambiente clínico.
Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components. It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images.
1 de feb. de 2019 · Toluidine blue O (TB) (C.I. 52040; mw = 305.83) is a thiazine cationic dye widely used as a biological stain since it has been discovered by William Perkin in 1856. This dye is soluble in water and in ethanol and reveals basophilia on substrates containing available anionic radicals.
- Benedicto de Campos Vidal, Maria Luiza S. Mello
- 2019
Toluidine blue O (C.I. 52040) for microscopy Certistain ® is adry dye that is used for the preparation of a staining solution for staining nuclei and for visualization of metachromasia.
27 de mar. de 2018 · Under the right circumstances, toluidine blue is a highly selective dye for detecting proteoglycans since the cationic dye requires a flexible anionic polymerized macromolecular structure with a high charge density in order to stain metachromatically.
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