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Fulvia fulva (sinónimo: Cladosporium fulvum ). Se han reportado muchas razas fisiológicas. Distribución. En todo el mundo. Síntomas. Los primeros síntomas aparecen como áreas que van de verde a amarillentas en la superficie posterior de las hojas más viejas.
El Cladosporium fulvum provoca la enfermedad llamada cladosporiosis. Esta enfermedad solo afecta a las hojas penetrando por los estomas en condiciones de humedad superiores a 70% y temperaturas entre 5 y 25º C. Puede sobrevivir en trozos de hojas secas sobre el suelo, en estructuras de invernadero, etc...
P. fulva no forma estructuras especializadas como haustorios, en su lugar, obtiene nutrientes a través del crecimiento micelial intercelular y la liberación de proteínas y péptidos que inducen en las células del hospedante la liberación de sustancias nutritivas.
Cladosporiosis, Mildiu, Oídiopsis y podredumbre gris son algunas de las principales enfermedades relacionadas con el cultivo del tomate. Syngenta te propone algunas soluciones al respecto.
- Isolates of Cladosporium fulvum
- Genomic DNA Extraction
- Genetic Diversity of C. fulvum Isolates
- PCR of Internal Transcribed Spacer
- PCR Amplification of AVR Genes and Race Identification
- PCR Amplification of ECP Genes
- Polymorphism Within AVR and ECP Genes
Eight new isolates of C. fulvum were obtained from tomato cultivars with typical leaf mould disease symptoms. They were collected in different locations of the two main tomato production areas of Argentina (Table 1). Monosporic cultures of isolates were maintained on potato dextrose agar (PDA) at 4 °C (Rollan et al. 2013), and deposited in the cult...
Total genomic DNA was extracted from monosporic cultures using the Wizard® Genomic DNA Purification kit. The quality and quantity of genomic DNA was evaluated by electrophoresis at 90 V for 30 min in a 0.7% (w/v) agarose gel that was stained with ethidium bromide (10% v/v). Gels were visualized using UV light and images were captured with the GeneS...
Diversity among the isolates was done by analysis of Inter Simple Sequence Repeat (ISSR) fingerprints. As outgroup S. lycopersici CIDEFI 216 was included in the analysis (Franco et al. 2017). Seven ISSR primers were used (AA5, AN, GA5, IA5, BA3, FA5, and LA5, Additional Table 1) to perform PCR amplifications as described by Franco et al. (2017). PC...
The ITS region was amplified by ITS-4 and ITS-5 primers (Additional Table 2, White et al. 1990), using fungal genomic DNA as template. Reaction mixtures contained 1× amplification buffer (Inbio Highway), 1.5 mM MgCl2(Inbio Highway), 40 ng of each primer, 200 mM each deoxynucleoside triphosphate (Inbio Highway), 25 ng of DNA template, and 1 U of Taq...
The primers used in the amplification reactions were those described by Stergiopoulos et al. (2007), which were modified since the universal M13 sequence was removed (Additional Table 2). PCRs were performed in a 15-μl volume with a thermocycler (Model Multigene gradient; Labnet International, In) programmed as follows: an initial denaturing step a...
Primers used in the reactions were those described by Stergiopoulos et al. (2007), except the ecp1 and ecp4 forward primers, which were modified to increase the specificity of the reaction (Additional Table 2). PCRs were performed in a 15-μl volume with a thermocycler (Model Multigene gradient; Labnet International, In) programmed as follows: an in...
Polymorphisms within avr and ecp genes of the 8 new isolates of C. fulvum as well as those of 12 isolates stored at the CIDEFI collection were identified by comparing nucleotide sequences as well as alignments of the PCR amplicons sequenced (Table 1). DNA sequences were aligned with ClustalW (Thompson et al. 1994) whereas multiple alignments of pre...
- César G. Lucentini, Roció Medina, Roció Medina, Mario E. E. Franco, Mario C. N. Saparrat, Pedro A. B...
- 2021
Moho de la hoja (Fulvia fulva) Los síntomas de esta enfermedad son manchas circulares y angulares de color amarillo en las hojas.
Agente causal: Passalora fulva (Cooke) U.Braun & Crous [syn. Cladosporium fulvum, Fulvia fulva] Taxonomía: Fungi > Dikarya > Ascomycota > Pezizomycotina > Dothideomycetes > Mycosphaerellales > Mycosphaerellaceae > Passalora. .
