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30 de ene. de 2023 · Just as a single polypeptide strand can self-fold into a complex 3D structure, a single strand of DNA can self-fold into DNA origami. Most DNA origami structures (i.e., the scaffold-staple and DNA tiling systems) utilize hundreds of short single-stranded DNA. As such, these structures come with challenges inherent to intermolecular construction. Many assembly challenges involving ...
Once single-stranded DNA is formed, the strand exchange protein RecA must coat that DNA in order for recombination to proceed (Figure 3; see RecA Protein and Homology). In in vitro assays the C-terminal domain of RecB, within the RecBCD holoenzyme, facilitates the loading of RecA protein preferentially onto the 3′-ended strand of DNA (relative to RecBCD entry; Churchill et al ., (1999) ).
The double-stranded DNA of the circular bacteria chromosome is opened at the origin of replication, forming a replication bubble. Each end of the bubble is a replication fork, a Y-shaped junction where double-stranded DNA is separated into two single strands. New DNA complementary to each single strand is synthesized at each replication fork.
DNA normally exists as a two antiparallel complementary strands held together by hydrogen bonds between adenines (A) and thymines (T), and between guanines (G) and cytosines (C). DNA is normally found as a double-stranded molecule in the cell whereas RNA is mostly single-stranded.
10 de ene. de 2022 · KAS-seq is an N3-kethoxal–assisted single-stranded DNA sequencing approach that allows rapid, sensitive and genome-wide mapping of single-stranded DNA produced in situ using as few as 1,000 ...
Single-strand breaks are the most common type of DNA damage that arise in cells. Keith Caldecott discusses the molecular mechanisms and organization of the pathways that repair these lesions and ...
26 de feb. de 2019 · Single-stranded DNA is a universal DNA damage signal 1,2,3. To investigate how the ssDNA signal is quantified, we studied the checkpoint response to a single site-specific DSB.
