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Loop-mediated isothermal amplification ( LAMP) is a single-tube technique for the amplification of DNA [1] and a low-cost alternative to detect certain diseases that was invented in 2000 at the University of Tokyo. [2] Reverse transcription loop-mediated isothermal amplification (RT-LAMP) combines LAMP with a reverse transcription ...
LAMP法 （らんぷほう、Loop-Mediated Isothermal Amplificationの略）とは、標的遺伝子の配列から6つの領域を選んで組み合わせた4種類の プライマー を用いて、鎖置換反応を利用して増幅させる方法である。 プライマーの設計によって、最初の増幅産物のプライマー結合部位にループ構造を生じるようにする。 ループ部分は一本鎖なので、次のプライマーが結合できる。 鎖置換活性の高い特殊な DNA合成酵素 は、進行方向にある二本鎖 DNA を解離しながら、自らの伸長反応を進めていく。 最終的には、もとの標的配列の約整数倍の長さの増幅産物が1時間ほどの65℃の反応で蓄積する。 したがって反応産物を電気泳動するとラダー状である。
Loop-Mediated Isothermal Amplification. Product Listing Application Overview. Loop-mediated isothermal amplification (LAMP) uses 4-6 primers recognizing 6-8 distinct regions of target DNA for a highly specific amplification reaction.
6 de abr. de 2020 · LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique.
24 de abr. de 2008 · Loop-mediated isothermal amplification (LAMP) is a simple, rapid, specific and cost-effective nucleic acid amplification method when compared to PCR, nucleic acid sequence-based...
3 de abr. de 2018 · Loop-mediated isothermal amplification (LAMP) is an isothermal nucleic acid amplification (iNAAT) technique known for its simplicity, sensitivity and speed. Its low-cost feature has resulted in ...
Loop-mediated isothermal amplification (LAMP), a newly developed gene amplification method, combines rapidity, simplicity, and high specificity. Several tests have been developed based on this method, and simplicity is maintained throughout all steps, from extraction of nucleic acids to detection of amplification.
