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En la biología molecular y la bioquímica, la transcriptasa inversa, también conocida como ADN polimerasa dependiente de ARN, es una enzima ADN polimerasa que transcribe, desde una sola cadena de ARN, una sola cadena de ADN.
A reverse transcriptase (RT) is an enzyme used to convert RNA genome to DNA, a process termed reverse transcription. Reverse transcriptases are used by viruses such as HIV and hepatitis B to replicate their genomes, by retrotransposon mobile genetic elements to proliferate within the host genome, and by eukaryotic cells to extend the ...
Los inhibidores de la transcriptasa inversa son fármacos que inhiben a la polimerasa de ADN dependiente de ARN y codificada por el VIH que convierte al ARN vírico en ADN provírico, que luego se incorpora en un cromosoma de la célula hospedadora. Esta polimerasa es conocida también como transcriptasa inversa.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR).
13 de mar. de 2024 · When the viral oncogene infects another cell, an enzyme called reverse transcriptase copies the single-stranded genetic material into double-stranded DNA, which is then integrated into the cellular genome.
10 de nov. de 2022 · Reverse transcriptases are DNA polymerases that can use RNA as a template for DNA synthesis. They thus catalyze the reverse of transcription. Although discovered in 1970, reverse transcriptases are still of great interest and are constantly being further developed for numerous modern research approaches.
Reverse transcriptases (both AMV and MoLV) complement DNA-dependent DNA polymerases (e.g., Pol Ik) in the dideoxynucleotide sequencing of DNA, especially at the regions of high GC content and/or secondary structures (108 ).
