Parece ser que fue la venganza de la Reina por haber asesinado a su secretario. El matrimonio de María el 15 de mayo del mismo año con James Hepburn, conde de Bothwell, que era el más evidente sospechoso del asesinato del duque de Albany, hizo aumentar los recelos y su impopularidad. 
James Stewart, 1st Earl of Moray (c. 1531 – 23 January 1570) was a member of the House of Stewart as the illegitimate son of King James V of Scotland. A supporter of his sister Mary, Queen of Scots , he was the regent of Scotland for his half-nephew, the infant King James VI , from 1567 until his assassination in 1570.
July 31 – Pope Paul IV authorizes the creation of the University of Douai (which will later become the University of Lille).  August 15 – Led by Don Tristán de Luna y Arellano , a Spanish missionary colony of 1,500 men, on 13 ships, arrives from Vera Cruz at Pensacola Bay , founding the oldest European settlement in the mainland U.S. ( St. Augustine is founded in 1565 .)
Marriage to Margaret Tudor. In 1509, Douglas married Margaret Hepburn, daughter of the Earl of Bothwell.After her death, and that of his father, in 1513, on 6 August 1514 the new Earl of Angus married the dowager queen and regent, Margaret Tudor, widow of James IV, mother of two-year-old James V, and elder sister of Henry VIII of England.
Isabel I ou Elizabeth I (Greenwich, 7 de setembro de 1533 – Richmond, 24 de março de 1603), também chamada de "A Rainha Virgem", "Gloriana" ou "Boa Rainha Bess" ("Bess" era como Roberto Durdley, seu favorito, a chamava) foi Rainha da Inglaterra e Irlanda de 1558 até sua morte e a quinta e última monarca da Casa de Tudor.
The next Orkney title was the dukedom of Orkney, which was given to James Hepburn, 4th Earl of Bothwell, husband of Mary, Queen of Scots, in 1567. Later that year, however, he forfeited the title when Mary was forced to abdicate. Earls of Orkney, Second Creation. The second earldom was created by James VI of Scotland.
François II, né à Fontainebleau le 19 janvier 1544 et mort à Orléans le 5 décembre 1560, est roi de France du 10 juillet 1559 jusqu'à sa mort.. Fils aîné d' Henri II et de Catherine de Médicis, il monte sur le trône de France à l'âge de quinze ans après la mort accidentelle de son père le 10 juillet 1559.