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CRISPOR is a program that helps design, evaluate and clone guide sequences for the CRISPR/Cas9 system.
- CRISPOR Manual
CRISPOR aligns the guides against the genome, reports those...
- CRISPOR Batch
Submit your sequence on the normal CRISPOR page, then use...
- Full List of Changes
CRISPOR Changes. Many thanks to the users that have...
- Crispor
is a program that helps design, evaluate and clone guide...
- Downloads/local Installation
A virtualBox virtual machine image for Windows/OSX/Linux...
- Downloads
A virtualBox virtual machine image for Windows/OSX/Linux...
- CRISPOR Manual
CRISPOR is a program that helps design, evaluate and clone guide sequences for the CRISPR/Cas9 system.
- Introduction
- New Features
- Plans For The Future
- Data Availability
- Acknowledgements
- Funding
Genome editing with the CRISPR–Cas system is impacting all fields of life sciences. While the technique is comparatively simple, cleavage specificity and efficiency depend primarily on the choice of the CRISPR guide sequence. To help with this step, various web tools find potential guide RNAs in an input sequence and rank them according to off-targ...
UCSC Genome Browser tracks
The UCSC Genome Browser provides interactive visualization of genome sequences and extensive annotations including gene exon-intron structures that is essential to design of genome editing experiments. As described in this year's NAR database issue (5), the UCSC Genome Browser now contains a track ‘CRISPR’ that shows pre-computed CRISPOR results for various model organisms and the spCas9 nuclease. The guides are coloured by efficiency score by default, details pages show the predicted off-tar...
Batch primer design for off-targets
In general, off-targets predicted by CRISPOR or any other tool are fortunately mostly false positives and only very few will be actually cleaved (4). To find these off-targets, the most sensitive assay is PCR followed by high-throughput amplicon sequencing. But designing dozens of primers can be cumbersome and the analysis of the results is not straightforward. To simplify primer design, we added a feature ‘Off-target primers’ from the ‘Cloning/PCR primers page’ that allow designing one pair...
Batch oligonucleotide design for gene knock-out screens and non-coding saturating mutagenesis screens
The most common application of CRISPOR are single guide experiments but increasingly, laboratories are conducting screens in cells using tens of thousands of guides. To make ordering of a library for a subset of several hundreds to thousands of genes easier, a new tool, CRISPOR Batch, accepts a list of genes and outputs a table with oligonucleotides ready to order from oligonucleotide pool suppliers, including subpool barcodes. It uses a list of existing, validated guide sequence libraries co...
We are planning to add support for base editors. Base editors consist of fusion of Cas9 nickase to cytidine or adenine deaminase that can convert C:G to T:A or A:T to G:C base pairs, respectively. Base editors can only convert bases that are within a small 5′ window of a suitable guide RNA target sequence and appear to possess different specifity r...
The complete source code of the CRISPOR website and a stand-alone command line version is available from GitHub (https://github.com/maximilianh/crisporWebsite/).
We thank Mark Diekhans for improving the performance, and Jean-Michel Heslan, Saulius Zuklys, Yueh-Chiang Hu, Peter Rogowsky, Pelin Özfiliz, Jitka Špačková and Alberto Stolfi for bug reports and feature requests.
California Institute of Regenerative Medicine [GC1R-06673-C to M.H.]; National Institutes of Health [5U54HG007990-04]; National Human Genome Research Institute [2U41HG002371-18]. Funding for open access charge: Chan-Zuckerberg Initiative grant 182809. Conflict of interest statement. None declared.
- Jean-Paul Concordet, Maximilian Haeussler
- 2018
A veces, los virus infectan a las bacterias. Muchas bacterias y arqueas tienen un sistema inmunológico para combatir estos fagos. Este sistema se llama "CRISPR" para abreviar. CRISPR es una forma en que las bacterias reconocen y destruyen el ADN del fago. Muchas personas contribuyeron al descubrimiento de CRISPR.
16 de sept. de 2015 · CRISPOR (http://crispor.org) is a computational sgRNA design tool that predicts off-target cleavage sites and offers a variety of on-target efficiency scoring systems to assist sgRNA...
