Margaret’s first pregnancy resulted in the birth of James, Duke of Rothesay at the Palace of Holyroodhouse in February 1507. However, this heir to the throne died a year later in February 1508. At this point Margaret was already pregnant with a second child, a daughter whose name is unknown, and who was born and died in July 1508.
James Stewart, 1st Earl of Moray (c. 1531 – 23 January 1570) was a member of the House of Stewart as the illegitimate son of King James V of Scotland. A supporter of his sister Mary, Queen of Scots, he was the regent of Scotland for his half-nephew, the infant King James VI, from 1567 until his assassination in 1570.
James was the third son of King James IV and his wife Margaret Tudor, the eldest daughter of Henry VII of England, and was the only legitimate child of James IV to survive infancy. He was born on 10 April 1512 at Linlithgow Palace and baptized the following day,  receiving the title Duke of Rothesay .
James I (late July 1394 – 21 February 1437) was King of Scots from 1406 until his assassination in 1437. The youngest of three sons, he was born in Dunfermline Abbey to King Robert III and Annabella Drummond. His older brother David, Duke of Rothesay, died under suspicious circumstances during detention by their uncle, Robert, Duke of Albany.
James was born on 19 June 1566 at Edinburgh Castle, and as the eldest son and heir apparent of the monarch automatically became Duke of Rothesay and Prince and Great Steward of Scotland. Five days later, an English diplomat Henry Killigrew saw the queen, who had not fully recovered and could only speak faintly.
On 26 March 1402 the Duke of Albany's nephew, David Stewart, Duke of Rothesay, had died in Falkland Palace while under his uncle's protection. King Robert III of Scotland, fearful that his younger son Prince James, the heir to the throne of Scotland, would suffer the same fate, sent him out of the kingdom to escape Albany's clutches.
James Francis Edward was born on 10 June 1688, at St. James's Palace. He was the son of James II of England and Ireland (VII of Scotland) and his second wife, Mary of Modena, both Roman Catholics. As first son of the reigning monarch he was automatically Duke of Cornwall and Duke of Rothesay, among other titles.