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16 de feb. de 2022 · Biology definition: Single-stranded DNA is a DNA molecule that consists of only a single strand contrary to the typical two strands of nucleotides in helical form. In nature, a single-stranded DNA genome can be found in Parvoviridae (class II viruses). Single-stranded DNA can also be produced artificially by rapidly cooling heat-denatured DNA.
1 de jul. de 2020 · We present an updated protocol for library preparation from single-stranded DNA, which is based on the splinted ligation of an adapter oligonucleotide to the 3′ ends of single DNA strands,...
- Marie-Theres Gansauge, Ayinuer Aximu-Petri, Sarah Nagel, Matthias Meyer
- 2020
Single-stranded DNA proteins (SSBs) bind ssDNA nonspecifically and are involved in all cellular pathways involving DNA manipulations. It is thought that their primary role is to protect ssDNA from nucleases as it arises during DNA replication, recombination, and repair.
21 de ene. de 2020 · Abstract. Methods for synthesizing arbitrary single-strand DNA (ssDNA) fragments are rapidly becoming fundamental tools for gene editing, DNA origami, DNA storage, and other applications. To meet the rising application requirements, numerous methods have been developed to produce ssDNA.
- Min Hao, Jianjun Qiao, Hao Qi
- 10.3390/genes11020116
- 2020
- Genes (Basel). 2020 Feb; 11(2): 116.
Single-stranded DNA-binding protein (SSB) is important not only for the protection of single-stranded DNA (ssDNA) but also for the recruitment of other proteins for DNA replication, recombination, and repair.
25 de abr. de 2018 · Single-stranded DNA (ssDNA) increases the likelihood of homology directed repair with reduced cellular toxicity.
8 de jul. de 2013 · HUH endonucleases catalyse breakage and joining of single-stranded DNA (ssDNA) by a unique mechanism using a Y motif Tyr to create a 5′ intermediate covalent bond with the ssDNA substrate.
