Resultado de búsqueda
Hace 3 días · The cells were smeared on a slide glass with Cytospin 4, fixed with pure methanol, and stained with 5% Giemsa staining solution. For toluidine blue staining, the cells were fixed with 3% glutaraldehyde and permeabilized with 0.5% Triton X-100, followed by toluidine blue staining. The cells were washed and observed under a microscope.
Hace 1 día · One micrometer thick coronal sections were stained with toluidine blue, and areas for ultrathin EM sectioning were selected. Ultrathin coronal sections (120 nm) were placed on silicon wafers and carbon-taped in aluminum stubs for subsequent SEM imaging. Cerebellar slice culture
Hace 5 días · Toluidine blue staining, LC-MS/MS analysis, qPCR, and immunoblotting were performed with AR and control mice. Results Ovalbumin-induced AR is diurnally rhythmic and associated with clock gene disruption in nasal mucosa.
Hace 2 días · However, toluidine blue staining does not positively stain mast cells in the ovine mammary gland, potentially due to differences in granule composition (Figure S5). Consequently, we have stained for c-Kit, a transmembrane tyrosine kinase receptor (Ribatti, 2018 ), that has been previously used to identify mast cells in human tissues (Lammie et al., 1994 ).
Hace 1 día · Ethylenediaminetetraacetic acid (EDTA), a classically used chelating agent of decalcification, maintains good morphological details, but its slow decalcification limits its wider applications.
Hace 4 días · Semithin sections (300 nm thickness) were obtained with a glass knife and stained with 1% (w/v) Toluidine Blue for cell localization and reorientation using a conventional optical microscope.
Hace 1 día · Nguyen et al. develop an approach to rapidly establish and screen cutaneous neurofibroma (cNF) organoids. These are benign tumors with no existing systemic therapy, exhibiting significant genetic and cellular heterogeneity. Patient-derived cNF organoids closely recapitulate cellular and molecular features of parental tumors and can be screened for drug discovery.
