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Hace 2 días · The cells were smeared on a slide glass with Cytospin 4, fixed with pure methanol, and stained with 5% Giemsa staining solution. For toluidine blue staining, the cells were fixed with 3% glutaraldehyde and permeabilized with 0.5% Triton X-100, followed by toluidine blue staining. The cells were washed and observed under a microscope.
Hace 3 días · In this randomized controlled trial, the researchers explored the impact of aPDT, utilizing a combination of toluidine blue O (TBO) and a red light-emitting diode (LED), on the formation of dental plaque. Positive Outcomes from the Study
Hace 3 días · To aid in the preliminary assessment of tissue morphology, sections with a thickness of 1 µm were stained with toluidine blue and examined using a BX41 light microscope (Olympus Co., Tokyo, Japan).
Hace 3 días · The samples were stained with Hematoxylin/Eosin (Leica Biosystems ®, Infinity 2.0) and Toluidine blue (Merk ®, Toluidine Blue O T-3260) for general assessment and identification of the proteoglycan content in the articular cartilage.
Hace 5 días · For chondrogenic induction, the cells were cultured in complete medium with 1% ITS-A solution (Gibco, Carlsbad, CA, USA) for 7–14 days and evaluated by a toluidine blue staining kit (Solarbio, Beijing, China) according to the manufacturer's instructions. 2.5. Gene silencing
Hace 1 día · Sections obtained with a rotary microtome (Leica® RM2245) were stained with 0.0 5% toluidine blue , mounted on water , and observed under a light microscope. Some fresh samples were hand-sectioned and stained with Sudan III [ 22 ] for the detection of lipophilic substances, and with ferric chloride and potassium dichromate for the detection of phenolic compounds [ 23 ].
Hace 21 horas · One micrometer thick coronal sections were stained with toluidine blue, and areas for ultrathin EM sectioning were selected. Ultrathin coronal sections (120 nm) were placed on silicon wafers and carbon-taped in aluminum stubs for subsequent SEM imaging. Cerebellar slice culture
